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SALSA® digitalMLPA Probemix D007 Acute Lymphoblastic Leukemia
D007 Acute Lymphoblastic Leukemia contains probes targeting:
- Genes and regions included in the well-established SALSA® MLPA® probemixes for ALL:
P335 ALL-IKZF1, P202 IKZF1-ERG, P327 iAMP21-ERG, and P329 CRLF2-CSF2RA-IL3RA.
- B-cell differentiation and cell cycle control genes, T-ALL-associated aberrations, iAMP21, 5q terminal
region and copy number aberrations of PAR1 region and other genes of emerging significance.
- Karyotyping probes for subtelomeric, pericentromeric and middle regions of chromosomal arms
to detect larger copy number alterations and ploidy status.
This assay is to be used with genomic DNA isolated from peripheral whole blood or bone marrow specimens and is not intended to be used with genomic DNA extracted from formalin-fixed paraffin-embedded or fresh-frozen tumour materials. CNAs detected with the D007 Acute lymphocytic leukemia Probemix should be confirmed with a different technique or with conventional MLPA probemixes when possible. particularly , CNAs detected by only one probe always require confirmation by another method. the bulk of defects in the genes included in this D007 Acute Lymphoblastic Leukemia Probemix are deletions, gains or amplifications, but point mutations can occur which can not be detected by digitalMLPA. it's therefore recommended to use this assay in combination with sequence analysis for the gene(s) of interest.
Features and Benefits
- 55 genes and regions targeted
- High dynamic range for copy number alteration (CNA) detection
- Only 20 ng of DNA input needed
- Quick turnaround of 48-72 hours
- Works on Illumina sequencing platform (all devices)
About
MRC-Holland Products in India
MRC Holland is a company based out of The Netherlands which has a proprietary technology - MLPA® (Multiplex Ligation-dependant Probe Amplification (MLPA®), a multiplex PCR method which enables detection of variation in copy number (CNV) of up to 60 DNA sequences in one reaction and can handle up to 96 DNA samples at a stretch with results being available within 24 hours.
Introduced in 2002, MLPA® is a gold standard in CNV detection and has proven to be a robust and easy-to-use techniques, and since it is highly sensitive to even a single nucleotide difference, it has applications in detection of point mutations.
MLPA®, when used in combination with a methylation-sensitive restriction enzyme, can be applied to detect DNA methylation patterns (MS_MLPA®). The first Melt Assay for spinal muscular atrophy (SMA) newborn screening was launched in 2018 and the first digitalMLPA® technique based assay was launched in 2020. digitalMLPA® combines the trusted quality of MLPA® with the power of next-generation sequencing to examine many more target sequences in a single reaction.
In diagnostics and research of both human genetic disorders and tumors, MLPA® is used in laboratories worldwide.
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Testimonials & Reviews
Dr. Chhaya Chande, Professor & HOD, Microbiology
GGMCJJ Hospitals, Mumbai
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Dr Sunil K Arora, Professor, Deptt of Immunopathology
PGIMER, Chandigarh
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MD, Spermprocessor Pvt Ltd
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Dr. Sreejesh S, Associate Professor, Dept of Hematology
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Dr Sudha S Murthy, Department of Pathology and Laboratory Medicine
BIACH & RI, Hyderabad
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Dr S Radhika MD, PhD
Professor, Deptt. Of Cytology & Gynaec Pathology, PGIMER, Chandigarh
“PGI Cytology Dept. has had a long association with DSS- Olympus Microscopy Division. They have provided excellent services- after sales service. The product is also of very good quality. We have had no problems with their products and services are of very good quality.”
Dr Nuzhat Husain
RMLIMS, Lucknow
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Dr Minu Singh
Assistant Professor, PGIMER, Chandigarh
“MRC Holland MLPA products provided by DSS are of good quality, have never faced any quality issues with their product or shipping condition. They provide prompt response upon any query.”
Mr. Krishnani Professor, SGPGI, Lucknow
“My experience with DSS so far has been excellent for the last 30 years- sales and service experience. Microscope products are very useful and sturdy with high precision.”