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PathoPlex RT-PCR kit
PathoPlex RT-PCR is a molecular diagnostic technique used for the qualitative detection of certain viral infections, specifically HIV (Human Immunodeficiency Virus), HBV (Hepatitis B Virus), and HCV (Hepatitis C Virus). This method utilizes reverse transcription polymerase chain reaction (RT-PCR) to amplify and detect specific genetic material (RNA in the case of HIV and HCV, and DNA for HBV) associated with these viruses.
The qualitative detection aspect means that the test can determine the presence or absence of the viral genetic material in a given sample. It is typically used as an initial screening tool to identify individuals who may be infected with HIV, HBV, or HCV.
The PathoPlex RT-PCR test is highly sensitive and specific, allowing for the accurate identification of these viruses. It is particularly useful in situations where early detection is critical, such as in the case of HIV, where early diagnosis and treatment can significantly improve patient outcomes.
The test is performed in a laboratory setting, and it involves several steps. Firstly, the viral RNA or DNA is extracted from the patient's blood or other appropriate samples. Then, the extracted genetic material is reverse transcribed into complementary DNA (cDNA) using an enzyme called reverse transcriptase. This cDNA is then amplified using the polymerase chain reaction (PCR) technique, which creates millions of copies of the specific viral genetic sequences if present in the sample. The amplified DNA is then analyzed using various detection methods, such as fluorescence-based techniques, to determine the presence or absence of the target viral genetic material.
It's important to note that while the PathoPlex RT-PCR test is highly accurate, it is primarily used for qualitative detection, meaning it can indicate if a person is infected with the virus but does not provide information about the viral load or the stage of the infection. Further confirmatory tests may be required for accurate diagnosis, monitoring, and treatment decisions.
If you have specific questions or require further information about PathoPlex RT-PCR or the detection of HIV, HBV, or HCV, please let me know.
Features and Benefits
- Kit Format: Multiplex, single-tube: Multiple detections in a single reaction
- Chemistry: TaqMan probe-based.
- MasterMix: Increased Inhibitor Tolerance, Enhanced Thermostability, Efficient PCR Amplification, and supports reverse transcription.
- Internal Control: RnaseP
- PCR Run Time: 90 minutes.
- Sample Compatability: Whole Human blood, Serum, & Plasma
- RT-PCR Instrumentation: Open platform kit compatible with 4 channels and above Instrument
About
Neodx Biotech Products in India
The term NeoDx denotes a new approach to Diagnostics. NeoDx Biotech Labs Pvt. Ltd. is a company that designs, develops & manufactures innovative molecular diagnostic products keeping in mind reliability, usability, accessibility & affordability. While NeoDx has been conceived as a company to create a portfolio of molecular diagnostic tests for both infectious and non-infectious diseases, the company has doubled down to develop and manufacture COVID19 related test kits (RNA Extraction Kits and the RT-PCR COVID19 test kits) in light of the Covid19 pandemic and the urgent need in the country for reliable and affordable RT-PCR test kits. NeoDx has now developed and launched an RNA extraction kit as well as the RT-PCR COVID test kit both of which they have received ICMR validation and approvals. These kits are now being manufactured under strict quality guidelines in a new state-of-the-art facility in Bangalore. Using the finest grade of raw materials and following rigid manufacturing and quality processes /protocols NeoDx ensures that the products manufactured in their facility are of the highest standards. The manufacturing site is ISO13485 certified and has started producing CE-IVD kits including Covid-19 RT PCR kits for all known variants including alpha, beta, gamma, delta and omicron.
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Testimonials & Reviews
Dr. Chhaya Chande, Professor & HOD, Microbiology
GGMCJJ Hospitals, Mumbai
“Ms. Megha Dhumal (Assistant Manager- Application) has done a satisfactory demonstration of the running of the Abbott Sample preparation machine model m2000sp and the Abbott RT-PCR machine model m2000rt. We appreciate the effort made by the DSS team under these difficult conditions to help our lab to carry out the imperative Covid-19 tests.”
Dr Sunil K Arora, Professor, Deptt of Immunopathology
PGIMER, Chandigarh
“We are using Confocal Microscope and one Fluorescence Microscope. Both are working fine. The after sales services by DSS have been excellent for functioning & upkeep of the microscopes. The applications support by experts from DSS is very useful. Keep it up!”
Dr Pramod Kumar Bajaj
MD, Spermprocessor Pvt Ltd
“Really excited to see the DSS Pathology solutions exhibition booth at APCON 2019 along with Magnus. We think all the upcoming technology had been displayed along with your efforts to make it Indigenous (Made in India) is highly appreciated. Wish you all the best. Keep it up!”
Dr. Sreejesh S, Associate Professor, Dept of Hematology
PGIMER, Chandigarh
“My experience with DSS so far has been very good till now. We are getting good support in both purchase as well as in troubleshooting. Very good experience with Mr Arun, Mr Manoj, Mr Mahesh and all others from the DSS team.”
Dr Sudha S Murthy, Department of Pathology and Laboratory Medicine
BIACH & RI, Hyderabad
“I am happy with DSS and associated with 19 years and use Dako antibody. Happy with Supply but need improvement.”
Dr S Radhika MD, PhD
Professor, Deptt. Of Cytology & Gynaec Pathology, PGIMER, Chandigarh
“PGI Cytology Dept. has had a long association with DSS- Olympus Microscopy Division. They have provided excellent services- after sales service. The product is also of very good quality. We have had no problems with their products and services are of very good quality.”
Dr Nuzhat Husain
RMLIMS, Lucknow
“Have been using Dako Reagants and Dako antibodies for a while. Services and products have been good and timely.”
Dr Minu Singh
Assistant Professor, PGIMER, Chandigarh
“MRC Holland MLPA products provided by DSS are of good quality, have never faced any quality issues with their product or shipping condition. They provide prompt response upon any query.”
Mr. Krishnani Professor, SGPGI, Lucknow
“My experience with DSS so far has been excellent for the last 30 years- sales and service experience. Microscope products are very useful and sturdy with high precision.”