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EGFR pharmDx Kits
Introduction
EGFR pharmDx Kit is a qualitative immunohistochemical assay. It includes all reagents necessary to identify the expression of epidermal growth factor receptor (EGFR) protein on the surface of normal and neoplastic cells. The kit will provide reproducible results with its’s standard protocol. EGFR is indicated as an aid in identifying colorectal cancer patients eligible for treatment with Erbitux® (cetuximab) or Vectibix™ (panitumumab).
The EGFR pharmDx™ Kit Includes:
- Proteinase K
- Peroxidase block
- Dako EGFR pharmDxTM Monoclonal Mouse Antibody
- Mouse IgG1 Negative Control Reagent
- Labeled Polymer-HRP
- DAB+ substrate buffer
- Liquid DAB+ Chromogen
- Dako Wash solution 10x
- Dako EGFR pharmDxTM Control slides
Procedure
The kit utilizes a simple two-step staining procedure and is suitable for formalin-fixed, paraffin-embedded specimens. Results can be available within 1 day, giving clinicians EGFR expression levels in a quick and reliable manner. This kit employs a ready-to-use visualization reagent based on dextran technology. A common dextran polymer backbone links both secondary goat anti-mouse antibody molecules and horseradish peroxidase molecules, thus eliminating the need for the sequential application of link antibody and peroxidase conjugate. Further, the addition of chromogen results in the formation of a visible reaction product at the antigen site. Later, the specimen may be counterstained and coverslipped.
Results Interpretation
Results are interpreted using a light microscope. Control slides containing two formalin-fixed, paraffin-embedded human cell lines with staining intensity scores of 2+ and 0 are provided for validation of the kit reagent performance.
The kit is available in Manual and Dako Automation configuration:
- K1492 EGFR pharmDx™ Kit for Manual Use for 35 Test
- K1494 EGFR pharmDx™ Kit for the Dako Autostainer Link for 50 Test
Features and Benefits
About
Dako Products in India
Agilent, a leader in life sciences, diagnostics and applied chemical markets, provides laboratories across the globe with instruments, services, consumables, applications and expertise that enable customers to gain the insights they seek. Agilents Diagnostics and Genomics Group markets Dako products to be a world leader in anatomic pathology & tissue-based cancer diagnostics. DSS has been representing this product line since 2007.
After acquisition of Dako by Agilent in 2012, the solutions provided have focused on Anatomic Pathology, Kits, Antibodies, Reagents, Specific Proteins, and Other Products, PharmacoDiagnostic®, Primary Antibodies, Visualization Systems, Multipurpose Antibodies, Secondary Antibodies, Control Reagents, Ancillaries, Clinical Immunochemistry, Flow Cytometry, and MHC Dextramers.
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Testimonials & Reviews
Dr. Chhaya Chande, Professor & HOD, Microbiology
GGMCJJ Hospitals, Mumbai
“Ms. Megha Dhumal (Assistant Manager- Application) has done a satisfactory demonstration of the running of the Abbott Sample preparation machine model m2000sp and the Abbott RT-PCR machine model m2000rt. We appreciate the effort made by the DSS team under these difficult conditions to help our lab to carry out the imperative Covid-19 tests.”
Dr Sunil K Arora, Professor, Deptt of Immunopathology
PGIMER, Chandigarh
“We are using Confocal Microscope and one Fluorescence Microscope. Both are working fine. The after sales services by DSS have been excellent for functioning & upkeep of the microscopes. The applications support by experts from DSS is very useful. Keep it up!”
Dr Pramod Kumar Bajaj
MD, Spermprocessor Pvt Ltd
“Really excited to see the DSS Pathology solutions exhibition booth at APCON 2019 along with Magnus. We think all the upcoming technology had been displayed along with your efforts to make it Indigenous (Made in India) is highly appreciated. Wish you all the best. Keep it up!”
Dr. Sreejesh S, Associate Professor, Dept of Hematology
PGIMER, Chandigarh
“My experience with DSS so far has been very good till now. We are getting good support in both purchase as well as in troubleshooting. Very good experience with Mr Arun, Mr Manoj, Mr Mahesh and all others from the DSS team.”
Dr Sudha S Murthy, Department of Pathology and Laboratory Medicine
BIACH & RI, Hyderabad
“I am happy with DSS and associated with 19 years and use Dako antibody. Happy with Supply but need improvement.”
Dr S Radhika MD, PhD
Professor, Deptt. Of Cytology & Gynaec Pathology, PGIMER, Chandigarh
“PGI Cytology Dept. has had a long association with DSS- Olympus Microscopy Division. They have provided excellent services- after sales service. The product is also of very good quality. We have had no problems with their products and services are of very good quality.”
Dr Nuzhat Husain
RMLIMS, Lucknow
“Have been using Dako Reagants and Dako antibodies for a while. Services and products have been good and timely.”
Dr Minu Singh
Assistant Professor, PGIMER, Chandigarh
“MRC Holland MLPA products provided by DSS are of good quality, have never faced any quality issues with their product or shipping condition. They provide prompt response upon any query.”
Mr. Krishnani Professor, SGPGI, Lucknow
“My experience with DSS so far has been excellent for the last 30 years- sales and service experience. Microscope products are very useful and sturdy with high precision.”