Abberior LIVE

Experience superior live-cell imaging with abberior LIVE dyes, meticulously crafted for STED (Stimulated Emission Depletion) and confocal microscopy. These innovative dyes set new benchmarks in live-cell labeling by delivering exceptional brightness and unmatched photostability. They simplify your research workflow, ensuring high-quality results every time.

Our range of ready-to-use probes is designed to meet various research needs, including DNA, tubulin, actin, and mitochondria labeling. Additionally, we offer specialized probes for advanced labeling techniques, such as click chemistry and SNAP-tag labeling, to enable versatile applications across your experiments. For unique research requirements, we can customize abberior LIVE dye conjugates tailored specifically to your needs.

Whether you are labeling Halo®-Tag fusion proteins or other targets in living cells, abberior LIVE dyes deliver outstanding performance. Preparing the necessary conjugates is straightforward: simply combine abberior LIVE carboxylic acid with commercially available Halo®-Tag amines to create reliable and efficient labeling solutions.

abberior LIVE 460L - The abberior LIVE 460L dye is specially designed for live-cell imaging, offering an extended Stokes shift of approximately 162 nm, ideal for both STED and confocal microscopy. With a broad excitation range of 440–490 nm and an emission band between 560–680 nm, this dye provides flexibility in multicolour imaging setups. In STED microscopy, it works seamlessly with STED laser wavelengths from 750–800 nm. These features make abberior LIVE 460L an ideal choice for multicolour live-cell STED experiments, especially as a third colour in combination with abberior LIVE 550 and LIVE 610.

abberior LIVE 510 - An advanced dye tailored for live-cell STED and confocal microscopy applications. Its unique molecular structure enhances fluorogenic properties, allowing efficient penetration across cell membranes. Ideal for researchers, this dye is optimally excited between 485-500 nm and, for STED applications, uses a depletion wavelength of 590-610 nm. It is an ideal choice for live-cell imaging, delivering outstanding clarity and reliability across various biological research applications.

abberior LIVE 550 - abberior LIVE 550 belongs to a new generation of fluorescent dyes for live-cell imaging, available exclusively from abberior. This highly photostable and intensely bright orange dye is optimized for excitation with a 561 nm laser. For STED imaging, a depletion wavelength in the 650-775 nm range is recommended.

The unique isomeric structure of abberior LIVE 550 enhances its cell permeability by stabilizing its preferred conformation. This allows the dye to be used in extremely low nanomolar concentrations, effectively minimizing background noise and cytotoxicity. It enables prolonged STED and confocal imaging of live cells and tissue with minimal interference.

For exceptional two-colour live-cell STED and confocal imaging, pair abberior LIVE 550 with abberior LIVE 610 for optimal results.

abberior LIVE SPY555 - Our HaloX® probes offer an efficient and flexible solution for labeling HaloTag® fusion proteins, whether in live cells or on cell surfaces. Designed with high affinity for the HaloTag® protein, these probes bind selectively while allowing dynamic exchange due to the absence of a covalent bond. This exchangeability enables the use of fresh probes as needed, which is particularly advantageous in confocal and STED microscopy. This transient interaction allows continuous detection of proteins in live cells over extended periods without the impact of photobleaching.

The abberior LIVE SPY555 HaloX® probe is a highly photostable, bright red-emitting dye, ideal for applications that use a 561 nm laser for excitation. For STED imaging, a recommended depletion wavelength between 750 - 800 nm ensures optimal performance.

This product is covered by one or more licenses from Spirochrome AG and, is intended for Research Use Only (RUO).

abberior LIVE 560 - The abberior LIVE 560 dye is specifically developed for live-cell imaging applications in STED and confocal microscopy. Its unique molecular structure enhances fluorogenicity and promotes efficient cell membrane penetration. Key features include:

• Optimal Excitation: Absorption maximum at 561 nm, excitable with a 561 nm laser.
• Recommended STED Wavelengths: Best results with depletion wavelengths between 650 - 775 nm.
• Live-Cell Imaging Versatility: Conjugated forms are ideal for imaging DNA molecules and the tubulin cytoskeleton in living cells.

Two-Color Imaging Compatibility: abberior LIVE 560 pairs seamlessly with abberior LIVE 610 for exceptional two-color live-cell imaging.

abberior LIVE ORANGE - abberior LIVE ORANGE mito is a specialized fluorescent probe designed for clear visualization of mitochondrial cristae using STED microscopy. Key features include:

• Targeted Staining: Specifically highlights mitochondrial cristae for detailed imaging.
• Long-lasting Imaging: Chemically structured for repeated excitation without toxicity, enabling extended observation of mitochondrial movement and substructures.
• Compatibility: Can be combined with other probes from the abberior LIVE series.
• Bright Fluorescence: Emits a vibrant orange signal, excited by a 561 nm laser. For STED, a 750-800 nm depletion wavelength is recommended.
• Product Details: Supplied as a lyophilized powder. Suggested stock solution: 1 mM in DMF or DMSO, with a working concentration of 100-500 nM, based on cell type or tissue.

abberior LIVE 590 - abberior LIVE 590 is a cutting-edge, bright orange fluorescent dye designed for live-cell imaging.

• Features high photostability, ideal for long-term imaging with minimal cytotoxicity.
• Excitable between 550 - 610 nm; for STED, a 750 - 800 nm depletion wavelength is recommended.
• Unique structure enhances cell permeability, effective at low nanomolar concentrations to reduce background interference.
• Perfect for extended STED and confocal imaging in living cells or tissues.

abberior LIVE 610 - abberior LIVE 610 is a cutting-edge red fluorescent dye designed for live-cell imaging. It offers:

• High photostability and bright fluorescence with excitation between 630-650 nm
• Ideal for STED imaging with a depletion wavelength of 750-800 nm
• Superior cell permeability allowing use in low concentrations, minimizing background and cytotoxicity
• Suitable for extended confocal and STED imaging in live cells or tissues
• Perfectly pairs with abberior LIVE 550 or LIVE 560 for enhanced 2-color imagingabberior LIVE RED - abberior LIVE RED is a next-generation red fluorescent dye for live-cell imaging, exclusively from abberior. Known for its high photostability and brightness, it is easily excitable with light between 630-650 nm, while a 750-800 nm wavelength is ideal for STED imaging. Its unique isomeric structure enhances cell permeability, enabling usage at low nanomolar concentrations that minimize background and cytotoxicity. abberior LIVE RED supports extended STED and confocal imaging for living cells or tissues and pairs perfectly with abberior LIVE 590, LIVE ORANGE, and LIVE 460L for optimal two- or three-colour live-cell imaging.abberior LIVE SiR - Our HaloX® probes enable specific labeling of HaloTag® fusion proteins on cell surfaces or within living cells. Designed for high affinity without forming covalent bonds, the probes allow a free exchange, making them ideal for long-term protein detection in confocal and STED microscopy. This transient binding helps mitigate bleaching, ensuring sustained visibility of proteins in live-cell imaging. The abberior LIVE SiR HaloX® probe, known for its brightness and photostability, emits a vivid red and is optimally excited with lasers in the 630-650 nm range. For STED, a depletion wavelength of 750-800 nm is recommended.

  This product is covered by one or more license from Spirochrome AG and, is intended for Research Use Only (RUO).