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RT-PCR

Real-time PCR has brought a significant transformation to clinical microbiology by enabling rapid and accurate detection of various microbial infections. Unlike traditional PCR, Real-time PCR provides real-time data during the amplification process, combining both detection and amplification into a streamlined, efficient solution.

This technique utilizes advanced fluorescent chemistries to monitor the accumulation of PCR products, offering high sensitivity and specificity. The advent of Real-time PCR and Real-time Reverse Transcription PCR (RT-PCR) has also played a crucial role in enhancing gene expression analysis.

Real-time PCR is suitable for both qualitative and quantitative applications. Selecting the right approach depends on a thorough understanding of the technology and its capabilities.

At DSS, we proudly represent global leaders like Abbott Molecular, NeoDx, and EntroGen—offering cutting-edge Real-time PCR solutions. These systems feature ready-to-use reagents, validated and optimized protocols, and intelligent, automated reagent handling to ensure accuracy, consistency, and ease of use.

RT-PCR
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Abbott RealTime HBV Assay

The Abbott RealTime HBV is an in vitro polymerase chain reaction (PCR) assay for the quantitation of Hepatitis B Virus (HBV) DNA in human plasma or serum from HBV-infected individuals.

Abbott Molecular Products, Applications & Specialities, Hepatitis, Infectious Diseases, Real Time PCR, RT-PCR, RT-PCR, Abbott Molecular, Clinical Diagnostics

Abbott RealTime HCV Assay

The Abbott RealTime HCV assay is an in vitro reverse transcription-polymerase chain reaction (RT-PCR) assay for the quantitation of hepatitis C viral ribonucleic acid (HCV RNA) in human serum and plasma from HCV-infected individuals.

Abbott Molecular Products, Applications & Specialities, Hepatitis, hepatitis A virus, Infectious Diseases, Real Time PCR, Real Time PCR, RT-PCR, RT-PCR, Abbott Molecular

Abbott RealTime HCV-GTII Assay

The Abbott RealTime HCV Genotype II is an in vitro reverse transcription-polymerase chain reaction (RT-PCR) assay for determining the genotype(s) of hepatitis C virus (HCV) in plasma and serum from HCV-infected individuals.

Abbott Molecular Products, Applications & Specialities, Hepatitis, hepatitis A virus, Infectious Diseases, olympus microscope, Real Time PCR, Real Time PCR, RT-PCR, RT-PCR, Abbott Molecular, Clinical Diagnostics

Abbott RealTime HIV-1 Assay

The Abbott RealTime HIV-1 assay is an in vitro reverse transcription-polymerase chain reaction (RT-PCR) assay for the quantitation of Human Immunodeficiency Virus type 1 (HIV-1) on the automated m2000 System in human plasma from HIV-1 infected individuals over the range of 40 to 10,000,000 copies/mL. The Abbott RealTime HIV-1 assay is intended for use in conjunction with clinical presentation and other laboratory markers for disease prognosis and for use as an aid in assessing viral response to antiretroviral treatment as measured by changes in plasma HIV-1 RNA levels.

Abbott Molecular Products, Applications & Specialities, hepatitis A virus, Infectious Diseases, olympus microscope, Pathology, Real Time PCR, Real Time PCR, RT-PCR, RT-PCR, Abbott Molecular, Clinical Diagnostics

Abbott RealTime HIV-1 Qualitative Assay

The Abbott RealTime HIV-1 Qualitative assay aids laboratories and physicians in diagnosing HIV-1 infections in pediatric and adult patients, providing usage of innovative DBS sampling and reliable detection of all HIV-1 groups and subtypes.

Abbott Molecular Products, Applications & Specialities, Infectious Diseases, olympus microscope, Real Time PCR, RT-PCR, RT-PCR, Abbott Molecular, Clinical Diagnostics

Abbott RealTime HPV Assay

Abbott RealTime High Risk HPV provides clinically proven and validated results allowing for improved and effi cient patient management in cervical cancer screening.

Abbott Molecular Products, olympus microscope, Real Time PCR, RT-PCR, RT-PCR, Abbott Molecular, Clinical Diagnostics

Abbott RealTime MTB Assay

The Abbott RealTime MTB assay is an in vitro polymerase chain reaction (PCR) for the qualitative detection of MTB

Abbott Molecular Products, Applications & Specialities, Infectious Diseases, MTB, olympus microscope, Pathology, Precision Medicine, Real Time PCR, Real Time PCR, RT-PCR, RT-PCR, Abbott Molecular, Clinical Diagnostics

Abbott RealTime MTB RIF/INH Resistance Assay

The Abbott RealTime MTB RIF/INH Resistance assay is an in vitro polymerase chain reaction (PCR) assay for the qualitative detection of Rifampicin (RIF) and Isoniazid (INH) resistance in MTB positive samples.

Abbott Molecular Products, Applications & Specialities, Infectious Diseases, MTB, olympus microscope, Pathology, Precision Medicine, Real Time PCR, Real Time PCR, RT-PCR, RT-PCR, Abbott Molecular, Clinical Diagnostics

BCR ABL Qualitative RT-PCR Kit – Major, Minor, Micro

Single-Tube, Multiplex Real-Time PCR Assay for sensitive and specific detection of Major (M-BCR) p210, Minor (m-BCR) p190, and Micro (μ-BCR) p230 BCR-ABL transcripts.

Applications & Specialities, Infectious Diseases, MLPA, olympus microscope, Precision Medicine, Real Time PCR, RT-PCR, RT-PCR, NeoDx, Clinical Diagnostics

BCR ABL Quantitative RT-PCR Kit – Major

IS quantitative, one-step RT-PCR for quantifying the IS ratios of BCR-ABL Major (M-BCR) p210 transcript in CML patient samples.

Applications & Specialities, Cancer Diagnostics Kit, Early cancer detection, Infectious Diseases, MLPA, olympus microscope, Pathology Lab Equipments, Precision Medicine, Real Time PCR, RT-PCR, RT-PCR, NeoDx, Clinical Diagnostics

BCR ABL Quantitative RT-PCR Kit – Major, Minor, Micro

Evaluates the molecular response up to 5 log reduction which is undetectable BCR-ABL1.

Applications & Specialities, Cancer Diagnostics Kit, Early cancer detection, Infectious Diseases, MLPA, olympus microscope, Precision Medicine, Real Time PCR, RT-PCR, RT-PCR, NeoDx, Clinical Diagnostics

Entrogen Real Time PCR Kit for Breast/Ovarian Cancer (RUO)

The PIK3CA gene encodes the p110a subunit of phosphatidyl 3-kinase, a lipid kinase involved in cell growth, proliferation, motility and survival. It has a central role in the PI3K-AKT-mTOR pathway. Somatic mutations in the PIK3CA gene have been implicated in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer.

Cancer Diagnostics Kit, Early cancer detection, Hospital Beds, Infectious Diseases, olympus microscope, Pathology Lab Equipments, Precision Medicine, Real Time PCR, RT-PCR, RT-PCR, ENTROGEN, Clinical Diagnostics

Entrogen REAL-TIME PCR GENOTYPING KIT

The UGT1A1 Genotyping Kit is a polymerase chain reaction (PCR)-based assay that uses allele-specific probes to identify the most common Irinotecan polymorphic variant.

Applications & Specialities, Cytogenetic Software, Infectious Diseases, olympus microscope, Pathology, Pathology Lab Equipments, Precision Medicine, Real Time PCR, Real Time PCR, RT-PCR, RT-PCR, ENTROGEN

Entrogen REAL-TIME PCR KITS FOR GASTRO-INTESTINAL STROMAL TUMORS (GIST)

EntroGen’s GIST mutation screening panel is a real-time polymerase chain reaction (PCR)-based assay that uses allele-specific primers to identify the presence of somatic mutations in c-KIT and PDGFRA genes.

Applications & Specialities, Infectious Diseases, olympus microscope, Precision Medicine, Products & Services, Real Time PCR, Real Time PCR, RT-PCR, RT-PCR, ENTROGEN

Entrogen REAL-TIME PCR KITS FOR LEUKEMIAS

EntroGen’s One-Step Detection kits provide reagents to detect and quantify the fusion gene transcripts listed below using total RNA isolated from blood or bone marrow.

Applications & Specialities, Infectious Diseases, olympus microscope, Precision Medicine, Real Time PCR, Real Time PCR, RT-PCR, RT-PCR, ENTROGEN

Entrogen REAL-TIME PCR KITS FOR LUNG CANCER

All kits require a real-time PCR instrument capable of detecting FAM and VIC fluorescent probes. Additionally, the Cell-Free EGFR Mutation Detection kit requires the capability to detect ROX and CY5 fluorescent probes.
All reagents required for PCR amplification/detection, as well as validated reaction controls, are included. Columns and reagents for DNA or RNA isolation are not included.

Applications & Specialities, Early cancer detection, ihc detection kit, Infectious Diseases, olympus microscope, Pathology Lab Equipments, Precision Medicine, Products & Services, Real Time PCR, Real Time PCR, RT-PCR, RT-PCR, ENTROGEN

Testimonials & Reviews

Dr. Chhaya Chande, Professor & HOD, Microbiology
Dr. Chhaya Chande, Professor & HOD, Microbiology
GGMCJJ Hospitals, Mumbai

“Ms. Megha Dhumal (Assistant Manager- Application) has done a satisfactory demonstration of the running of the Abbott Sample preparation machine model m2000sp and the Abbott RT-PCR machine model m2000rt. We appreciate the effort made by the DSS team under these difficult conditions to help our lab to carry out the imperative Covid-19 tests.”

Dr Sunil K Arora, Professor, Deptt of Immunopathology
Dr Sunil K Arora, Professor, Deptt of Immunopathology
PGIMER, Chandigarh

“We are using Confocal Microscope and one Fluorescence Microscope. Both are working fine. The after sales services by DSS have been excellent for functioning & upkeep of the microscopes. The applications support by experts from DSS is very useful. Keep it up!”

Dr Pramod Kumar Bajaj
Dr Pramod Kumar Bajaj
MD, Spermprocessor Pvt Ltd

“Really excited to see the DSS Pathology solutions exhibition booth at APCON 2019 along with Magnus. We think all the upcoming technology had been displayed along with your efforts to make it Indigenous (Made in India) is highly appreciated. Wish you all the best. Keep it up!”

Dr. Sreejesh S, Associate Professor, Dept of Hematology
Dr. Sreejesh S, Associate Professor, Dept of Hematology
PGIMER, Chandigarh

“My experience with DSS so far has been very good till now. We are getting good support in both purchase as well as in troubleshooting. Very good experience with Mr Arun, Mr Manoj, Mr Mahesh and all others from the DSS team.”

Dr Sudha S Murthy, Department of Pathology and Laboratory Medicine
Dr Sudha S Murthy, Department of Pathology and Laboratory Medicine
BIACH & RI, Hyderabad

“I am happy with DSS and associated with 19 years and use Dako antibody. Happy with Supply but need improvement.”

Dr S Radhika MD, PhD
Dr S Radhika MD, PhD
Professor, Deptt. Of Cytology & Gynaec Pathology, PGIMER, Chandigarh

“PGI Cytology Dept. has had a long association with DSS- Olympus Microscopy Division. They have provided excellent services- after sales service. The product is also of very good quality. We have had no problems with their products and services are of very good quality.”

Dr Nuzhat Husain
Dr Nuzhat Husain
RMLIMS, Lucknow

“Have been using Dako Reagants and Dako antibodies for a while. Services and products have been good and timely.”

Dr Minu Singh
Dr Minu Singh
Assistant Professor, PGIMER, Chandigarh

“MRC Holland MLPA products provided by DSS are of good quality, have never faced any quality issues with their product or shipping condition. They provide prompt response upon any query.”

Mr. Krishnani
Mr. Krishnani Professor, SGPGI, Lucknow

“My experience with DSS so far has been excellent for the last 30 years- sales and service experience. Microscope products are very useful and sturdy with high precision.”